Susana Mendez

Cornell University | Baker Institute
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What is Leishmaniasis | Life Cicle | History | Clinical Presentations | Canine Leishmaniasis
Susana Mendez | Wenhui Wu | Meleana Hinchman | Lu Huang | David Byun | Eliese Freidel | Patricia Green | Sandfly Kids
Parasitology Links | Leishmaniasis Links | Cornell Links | Other Links
Leishmania Vaccine | Immunecomplex | Hookworm | Goat | Sandfly
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Publications

1. Bower MA, Constant SL, Mendez S. “The Na-ASP2 protein secreted by the infective larvae of the human helminth Necator americanus. Experimental Parasitology  (in press)

2. Mendez S, Hatem CL, Kesavan AK, Lopez-Molina J, Pitt MLM, Dannenberg Jr. AM, Manabe YC. Further studies in inbred and outbred New Zealand white rabbits: Cellular composition of Tuberculous granulomas and implications for disease containment. Veterinary Immunology (in press).

3. Manabe YC, Kesavan AK, Lopez-Molina J, Hatem CL, Brooks M, Fujiwara R, Hochstein K, Pitt MLM, Tufariello J, Chan J, McMurray DN, Dannenberg Jr. AM, Mendez S. The aerosol rabbit model of tuberculosis latency, reactivation and immune reconstitution inflammatory syndrome. Tuberculosis (in press).

4. Mendez S, Samuel AD, Antoine AD, Ahn S, Hotez PJ (2007). Use of the air pouch model of investigate immune responses to a hookworm vaccine containing the Na-ASP-2 protein in rats. Parasite Immunology 30:53-56.

5. Fujiwara RT, Zhan B, Mendez S, Loukas A, Bueno L, Wang Y, Plieskatt J, Oksov Y, Lustigman S, Bottazzi ME, Hotez PJ, Bethony JM. (2007). Reduction of worm fecundity and canine host blood loss mediates protection against hookworm infection elicited by vaccination with recombinant Ac-16. Clinical and Vaccine Immunology 14(3):281-7.

6. Wu W, Weigand L, Belkaid Y, Mendez S. (2006). Immunomodulatory effects associated with a live vaccine against Leishmania major containing CpG oligodeoxynucleotides. European Journal of Immunology 36(12):3238-47.

7. Fujiwara RT, Geiger S, Bethony S, Mendez S (2006). Comparative immunology of human and animal models of hookworm infection (review). Parasite Immunology 28(7):285-93.

8. Ghosh K, Wu W, Antoine AD, Bottazzi ME, Valenzuela JG, Hotez PJ, Mendez S. The impact of concurrent and treated Ancylostoma ceylanicum hookworm infections on the immunogenicity of a recombinant hookworm vaccine in hamsters. The Journal of Infectious Disease, 193(1):155-62.

9. Fujiwara RT, Bethony J, Bueno LL, Wang Y, Ahn SY, Samuel AD, Bottazzi ME, Hotez PJ, Mendez S (2005). Immunogenicity of the hookworm Na-ASP-2 vaccine candidate: Characterization of humoral and cellular responses after vaccination in the Sprague Dawley rat. Human Vaccines, 1(3): 123-128. 

10. Loukas A, Bethony JM, Mendez S, Fujiwara RT, Goud GN, Ranjit N, Zhan B, Jones K, Bottazzi ME, Hotez PJ (2005). Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs. PLoS Medicine, 2(10):e295.

11. Kesavan AK, Mendez S, Hatem CL, Lopez-Molina J, Aird K, Pitt ML, Dannenberg AM Jr, Manabe YC (2005). Effects of dexamethasone and transient malnutrition on rabbits infected with aerosolized Mycobacterium tuberculosis CDC1551. Infection and Immunity, 73 (10):7056-60.

12. Zhan B, Liu S, Perally S, Xue J, Fujiwara R, Brophy P, Xiao S, Liu Y, Feng J, Williamson A, Wang Y, Bueno LL, Mendez S, Goud G, Bethony JM, Hawdon JM, Loukas A, Jones K, Hotez PJ (2005). Biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm Ancylostoma caninum. Infection and Immunity, 73(10):6903-11.

13. Bertholet S, Debrabant A, Afrin F, Caler E, Mendez S, Tabbara KS, Belkaid Y, Sacks DL (2005). Antigen requirements for efficient priming of CD8+ T cells by Leishmania major-infected dendritic cells. Infection and Immunity,73(10):6620-8.

14. Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM. Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs (2005). Vaccine 24(4):501-9.

15. Goud GN, Bottazzi ME, Zhan B, Mendez S, Deumic V, Plieskatt J, Liu S, Wang Y, Bueno L, Fujiwara R, Samuel A, Ahn SY, Solanki M, Asojo OA, Wang J, Bethony JM, Loukas A, Roy M, Hotez PJ (2005). Expression of the Necator americanus hookworm larval antigen Na-ASP-2 in Pichia pastoris and purification of the recombinant protein for use in human clinical trials. Vaccine 23(39):4754-64.

16.Tabbara KS, Peters NC, Afrin F, Mendez S, Bertholet S, Belkaid Y, Sacks DL (2005). Conditions influencing the efficacy of vaccination with live organisms against Leishmania major infection. Infection and Immunity;73(8):4714-22.

17.Bethony J, Loukas A, Smout M, Brooker S, Mendez S, Plieskatt J, Goud G, Bottazzi ME, Zhan B, Wang Y, Williamson A, Lustigman S, Correa-Oliveira R, Xiao S, and Hotez PJ (2005). Antibodies against a secreted protein from hookworm larvae reduce the intensity of hookworm infection in humans and vaccinated laboratory animals. FASEB Journal. 2005 Jul 21.

18. Mendez S, Valenzuela JG, Wu W, and Hotez PJ. (2005) Host cytokine production, lymphoproliferation, and antibody responses during the course of Ancylostoma ceylanicum infection in the Golden Syrian hamster. Infection and Immunity. 2005 Jun;73(6):3402-7.

19. Mendez S, Zhan B, Goud G, Ghosh K, Dobardzic D, Wu W, Liu S, Deumic V, Dobardzic R, Liu Y, Bethony J, Hotez PJ.Effect of combining the larval antigens Ancylostoma Secreted Protein 2 (ASP-2) and metalloprotease 1 (MTP-1) in protecting hamsters against hookworm infection and disease caused by Ancylostoma ceylanicum(2005). Vaccine, 23(24):3121-30.

20. Anderson CF, Mendez S, Sacks DL (2005). Nonhealing Infection despite Th1 Polarization Produced by a Strain of Leishmania major in C57BL/6 Mice. The Journal of  Immunology, 174(5):2934-41.

21. Mendez S, Reckling SK, Piccirillo CA, Sacks D, Belkaid Y (2004). Role for CD4 (+) CD25(+) regulatory T cells in reactivation of persistent leishmaniasis and control of concomitant immunity. The Journal of Experimental Medicine, 200(2):201-10.

22. Loukas A, Bethony JM, Williamson AL, Goud GN, Mendez S, Zhan B, Hawdon JM, Elena Bottazzi M, Brindley PJ, Hotez PJ (2004). Vaccination of dogs with a recombinant cysteine protease from the intestine of canine hookworms diminishes the fecundity and growth of worms. The Journal of Infectious Diseases, 189(10):1952-61.

23. Goud GN, Zhan B, Ghosh K, Loukas L, Hawdon J, Dobardzic A, Deumic V, Liu S, Dobardzic D, Zook BC, Jin Q, Liu Y, Hoffman L, Chung-Debose S, Patel R, Mendez S  and Hotez PJ. Cloning, Yeast Expression, Isolation and Vaccine Testing of Recombinant Ancylostoma secreted protein 1 (ASP-1) and ASP-2 from Ancylostoma ceylanicum. The Journal of Infectious Diseases, 189(5):919-29.

24.Hotez PJ, Zhan B,  Bethony JM, Loukas A, Williamson A, Goud GN, Hawdon JM, Dobardzic A, Dobardzic R, Ghosh K, Bottazzi ME, Mendez S, Zook B, Wang Y, Liu S, Essiet-Gibson I, Chung-Debose S, Xiao S, Knox D, Meagher M, Inan M, Correa-Oliveira R, Vilk P, Shepherd HR, Brandt W and Russell, PK. Progress in the development of a recombinant vaccine for human hookworm disease: The Human Hookworm Vaccine Initiative. International Journal for Parasitology, 33(11):1245-58.

25. Zhang WW, Mendez S, (ex equo) Ghosh A, Myler P, Ivens A, Clos J, Sacks DL, Matlashewski G (2003).Comparison of the A2 gene locus in L. donovani and L. major and its control over cutaneous infection.Journal of Biological Chemistry 278(37):35508-15.

26. Mendez S, Tabbara K, Belkaid Y, Bertholet S, Verthelyi D, Klinmann D, Seder RA, Sacks DL (2003). Improving the safety of a live, non-attenuated vaccine against cutaneous leishmaniasis by co-injection of CpG ODNs. Infection and Immunity, 71(9):5121-5129

27. Freidag BL, Mendez S, Cheever AW, Kenney RT, Flynn B, Sacks DL, Seder RA (2003). Immunological and pathological evaluation of rhesus macaques infected with Leishmania major. Experimental Parasitology,103(3-4):160-8.

28. Shah JA, Darrah PA, Ambrozak DR, Turon TN, Mendez S, Kirman J, Wu CY, Glaichenhaus N, Seder RA (2003). Dendritic cells are responsible for the capacity of CpG Oligodeoxynucleotides to act as an adjuvant for protective vaccine immunity against Leishmania major in mice. The Journal of Experimental Medicine,198(2):281-91.

29. Fernandez-Perez FJ, Gomez-Munoz MT, Mendez S, Alunda JM (2003).Leishmania-specific lymphoproliferative responses and IgG1/IgG2 immunodetection patterns by Western blot in asymptomatic, symptomatic and treated dogs.Acta Tropica.;86(1):83-91.

30. Belkaid Y, Piccirillo CA, Méndez S, Shevach EM, Sacks DL (2003). CD4+CD25+ immunoregulatory T cells control Leishmania major persistence and the development of concomitant immunity. Nature, 420(6915):502-7.

31. Mendez S, Belkaid, Y, Seder RA,  Sacks, D (2002). Optimization of DNA vaccination against cutaneous leishmaniasis. Vaccine 20:3702-3708.

32. Rhee EG, Mendez S (ex equo), Shah JA, Kirman J, Turon T, Davey D, Davis H, Klinmann D, Sacks DL,  Seder RA (2002). Vaccination with leishmanial antigen and CpG Oligodeoxynucleotides induces long term CD4 and CD8+ T cell responses and protection against Leishmania major infection. The Journal of Experimental Medicine, 195(12): 1565-1573.

33. Belkaid Y, von Stebut E, Mendez S, Lira R, Udey MC, Sacks DL (2002). CD8+ T cells are required for pathogenesis and immunity in C57Bl/6 mice following low dose, intra-dermal challenge with Leishmania major. The Journal of Immunology, 168(8):3992-4000.

34. Gómez-Muñoz, MT, Gómez-Iglesias, LA, Fernández-Pérez, FJ, Méndez S, Domínguez, IA, de la Fuente, C, Alunda, JM, Cuquerella, M (2002). Experimental Haemonchus contortus infection and challenge in the Churra sheep breed: immunological and pathophysiological parameters. Revista  Iberica de Parasitologia, 61(3-4):83-89.

35. Méndez S, Alunda JM (2001). Leishmania infantum: in vitro effect of some pentamidine-derivatives on amastigotes cultured in J774.G8 cells. In: Canine leishmaniasis: treatment and follow-up. Cost B9 Expert Meeting, Madrid, pp: 21.

36. Udey MC, von Stebut E, Mendez S, Sacks DL, Belkaid Y (2001). Skin dendritic cells in murine cutaneous leishmaniasis. Immunobiology 204, 590-594.

37. Méndez S, Fernández-Pérez F, Santín M, de la Fuente C, Cuquerella M, Gómez-Muñoz MT, Alunda JM (2001). Correlation between in vivo and in vitro infectivity of Leishmania infantum clones. The Journal of Eukaryotic Microbiology 48(6): 616-621.

38. Belkaid,Y, Hoffman KF, Mendez S, Kamhawi S, Udey MC, Wynn TA, Sacks DL (2001).The role of interleukin (IL)-10 in the persistence of Leishmania major in the skin after healing and the therapeutic potential of anti IL-10 receptor antibody for sterile cure. The Journal of Experimental Medicine, 194:1497-1506.

39. Valenzuela JG, Belkaid Y, Garfield M, Mendez S, Kamhawi S, Rowton E, Sacks DL, Ribeiro J MC (2001). Toward a defined anti-Leishmania vaccine targeting vector salivary antigens: characterization of a protective salivary protein. The Journal of Experimental Medicine, 194 (3): 331-342.

40. Mendez S, Gurunathan S, Kamhawi S, Belkaid Y, Moga MA, Skeiky YAW, Campos-Neto A, Reed S, Seder RA, Sacks DL (2001). The potency and durability of DNA- and protein-based vaccines against Leishmania major evaluated using low dose, intra-dermal challenge. The Journal of Immunology, 166: 5122-5128.

41. Belkaid Y, Mendez S, Lira R, Kadambi N, Milon G, Sacks D (2000). A natural model of Leishmania major infection a prolonged “silent” phase amplification in the skin before the onset of lesion formation and immunity. The Journal of Immunology, 165: 969-977.

42. Gómez-Muñoz MT, Domínguez IA, Gómez-Iglesias LA, Fernández-Pérez FJ, Méndez S, de la Fuente C, Alunda JM, Cuquerella M (2000).  Serodiagnosis of haemonchosis with a somatic antigen (Hc26) in several breeds of sheep. Journal of Veterinary Diagnosis Investigation. Jul;12(4):354-60

43. Domínguez-Toraño IA, Cuquerella M, Gómez-Muñoz MT, Méndez S, Fernández FJ, Alunda, JM (2000). Vaccination of Manchego lambs against Haemonchus contortus with a somatic fractions (p26/23) of adults parasites. Parasite Immunology 22(3):131-138.

44. Mendez S, Nell M, Fernandez-Perez FJ, Alunda JM (1999). Sensitivity of Leishmania infantum amastigotes to fluorinated L-ornithine analogues. Medical Science Research, 27:87-89.

45. Fernández-Pérez FJ, Méndez S, de la Fuente C, Cuquerella M, Gómez MT, Alunda JM (1999). Value of western blot in the clinical follow-up of canine leishmaniasis. Journal of Veterinary Diagnostic Investigation 11:170-173.

46. Lira R, Mendez S, Carrera L, Jaffe C, Neva F, Sacks, D (1998). Leishmania tropica: The identification and purification of metacyclic promastigotes and use in establishing  mouse and hamster models of cutaneous and visceral disease. Experimental Parasitology, 89 (3): 331-342.

47. Méndez S, Nell M, Fernández-Pérez FJ, Alunda JM (1998). Sensitivity of Leishmania infantum amastigotes to polyamines analogues. Reversion by exogenous putrescine. Medical Science Research 27: 87-89.

48. Mendez S, Fernández-Pérez F, de la Fuente C, Cuquerella M, Gómez MT, Alunda JM (1998). Partial anaerobiosis induces infectivity or Leishmania infantum promastigotes. Parasitology Research 85(6): 507-509

49. Fernández-Pérez FJ, Méndez S, de la Fuente C, Gómez-Muñoz MT, Cuquerella M, Alunda JM (1998). Improved diagnosis and follow-up of canine leismaniasis using amastigote-based indirect immunoflourescence. American Journal of Tropical Medicine and Hygiene 61(4):652-3.

50. Gómez-Muñoz MT, Cuquerella M, Domínguez IA, Gómez Iglesias LA, Fernández-Pérez FJ, Méndez S, de la Fuente C, Alunda, JM (1998). Serum antibody response of Castellana sheep to Haemonchus contortus infection and challenge. Relationship to abomasal worm burdens. Veterinary Parasitology  81(4): 281-289.

51. Méndez S, Nell M, Alunda JM (1996). Leishmania infantum: infection of macrophages in vitro with promastigotes. International Journal for Parasitology 26: 619-622.

52. Carrera L, Fermín ML, Tesouro M, García P, Rollán E, González JL, Méndez S, Cuquerella M, Alunda,JM (1996). Antibody response in dogs experimentally infected with Leishmania infantum: infection course and antigen markers. Experimental Parasitology 82: 139-146.

53. Cuquerella M, Gómez-Muñoz MT, Méndez S, Alunda JM (1994). Partial protection of Manchego sheep against Haemonchus contortus after a 6-month postpriming period. Preliminary note. J. Vet. Med. B. 41:399-406.

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